Shikonin Selectively Inhibits Splicing of Tumor Necrosis Factor‐alpha pre‐mRNA

Aspirin inhibits tumor necrosis factoralpha gene expression in murine tissue macrophages.

Aspirin has been reported to inhibit the activation of nuclear factor-kappaB (NF-kappaB) through stabilization of inhibitor kappaB (IkappaB). This observation led us to investigate the role of aspirin in suppressing the activation of the NF-kappaB-regulated tumor necrosis factor-alpha (TNF-alpha) gene expression in primary macrophages. We now report that therapeutic doses of aspirin suppress li...

Inhibition of tumor necrosis factor-alpha through selective blockade of Pre-mRNA splicing by shikonin.

We previously developed a gene-gun-based in vivo screening system and identified shikonin as a potent suppressor of tumor necrosis factor-alpha (TNF-alpha) gene expression. Here, we show that shikonin selectively inhibits the expression of TNF-alpha at the RNA splicing level. Treatment of lipopolysaccharide-stimulated human primary monocytes and THP-1 cells with shikonin resulted in normal tran...

Thalidomide Selectively Inhibits Tumor

Thalidomide selectively inhibits the production of human monocyte tumor necrosis factor a (TNF-a) when these cells are triggered with lipopolysaccharide and other agonists in culture. 40% inhibition occurs at the clinically achievable dose of the drug of 1 Ag/ml . In contrast, the amount of total protein and individual proteins labeled with [31S]methionine and expressed on SDS-PAGE are not infl...

Azithromycin Selectively Reduces Tumor Necrosis Factor

1 CYSTIC FIBROSIS AIRWAY EPITHELIAL CELLS. 2 3 Running title: Azithromycin reduces TNF-α in cystic fibrosis cells. 4 Cristina Cigana, Baroukh Maurice Assael and Paola Melotti* 5 Cystic Fibrosis Center, Azienda Ospedaliera di Verona, Verona, ITALY. 6 7 *Corresponding author. Mailing address: Cystic Fibrosis Center Azienda Ospedaliera di 8 Verona, piazzale Stefani, 1 37126 Verona, Italy. Phone: +...

Erythromycin Inhibits Tumor Necrosis Factor